Yes. Scout comments on Boo Radley's gray eyes towards the end of chapter 29.
Boo Radley remains a mysterious character for the majority of Scout and Jem's childhood but comes to their rescue in chapter 28 when Bob Ewell viciously attacks them. After Boo saves the children and kills Bob Ewell, he carries Jem home, where Dr. Reynolds arrives to take care of him.
At the end of chapter 29, Scout finally sees Arthur "Boo" Radley for the first time in Jem's bedroom and begins to describe his appearance by mentioning that he has extremely white skin. Scout also mentions that Boo Radley has hollow cheeks and a wide, thin mouth. Scout then comments on Boo Radley's gray eyes, which are so colorless that she assumes he is blind. In addition to his white skin, gray eyes, and gaunt appearance, Scout also notices his feathery, dead hair. After examining Boo standing quietly in the corner of Jem's room, Scout's eyes begin to tear up as she says, "Hey, Boo" (Lee, 274).