Act Two is one of the most significant acts of Romeo and Juliet. In the act, the love between Romeo and Juliet is developed. The prologue sets up their romantic encounter and the two lovers first see each other at the Capulets' party. The famous balcony scene is in this act (Act Two, Scene Two), Romeo decides he wants to marry Juliet, and the two lovers ultimately marry. Indeed, the plot advances more rapidly in this act than perhaps any other act in the play. Despite being teenagers, Romeo and Juliet meet, fall in love, and marry in only one act.
If a "loss of innocence" is located in the second act - and it can be argued that Romeo and Juliet lose their innocence in a different act - then it probably occurs around the marriage of these characters. Friar Lawrence suggests the two wait in their marriage ("These violent delights have violent ends," II.vi.9), but Romeo and Juliet do not listen to his warnings. Instead, they rush firmly into marriage. By the end of the act, it can be argued that the two have progressed into being adults, and their actions will have mature consequences. If the loss of innocence is presented in this act, then it is presented as something that can be easily cast off.