The climax of Rabbit Hole could be said to occur in the first scene of the second act, when Jason visits Howie's home and Howie demands that he leave. Jason is a seventeen-year-old young man who accidentally hit Howie's child while driving, killing him.

This climatic scene between Jason and Howie is the only scene involving direct explicit conflict between characters onstage. As Jason stands at the door of Howie's home awaiting entrance, Howie lashes out in anger and resentment. The tone of the play is dark and fatalistic. Jason really isn't at fault for the death of Howie's child; a number of events conspired together to produce this tragic event. At the end of the play, Becca (Howie's wife) speculates about the implications of the multiverse theory. She finds solace in the thought that perhaps in another universe, their family is happy.