The characters of Comus by John Milton are as follows: the senior god Jove, who presides over the eternal realm of the classical gods.

Jove sends the attendant Spirit to defend virtue on earth when it is under threat. The attendant Spirit guides and protects the lost siblings in the dark woods, instructs and leads the brothers to where Comus has lured their sister, he protects them from harm and invokes the Nymph Sabrina to free their virtuous sister.

The Elder Brother and the Younger Brother search for their sister in the dark wood. The Elder Brother provides guidance to the Younger Brother on how virtue is protected from evil, and to focus on hope given the choice of hope or despair. Their father is king of the land.

The Lady gets separated from her brothers at night in the woods where she is deceived by Comus to follow him. Comus's objective is to get her to drink from his cup and turn her into an animal-headed slave of her base passions. She rejects his arguments and recognizes him as a deceiver.

Comus is the son of the wine god Bacchus and the sorceress Circe. He is more powerful than Circe in his black magic, and he turns men and women that drink from his cup into animal-headed monsters with human bodies that forget their previous lives, do not recognize their fallen condition, and think they are more attractive than ever. They accompany Comus in his sensual revels.

The water Nymph Sabrina is a protectress of chastity and virtue. She comes when invoked by the attendant Spirit and her words free the Lady from the sorcerer Comus's enchantment.