A further example of irony comes in the fact that Aylmer's magic elixir does indeed remove his wife's birthmark as intended, but also ends up killing her. The whole point of this weird experiment was to ensure that Georgiana would conform to her husband's strict ideas of what constitutes beauty. Yet ironically Aylmer's potion has destroyed Georgiana's physical beauty in an effort to improve it. Thanks to Aylmer's ridiculously high standards of beauty, his wife will never be beautiful again, or anything else, for that matter.

An additional irony is that Aylmer, though undoubtedly a highly intelligent man, is not very wise. If he'd had sufficient wisdom he would've accepted Georgiana for what she was, birthmark and all, instead of trying to change her appearance by such drastic measures.

In my opinion, the main irony in this story has to do with Aylmer and Aminadab.  The irony is that Aminadab knows way better than Aylmer what is going to happen.   This is ironic because Aylmer is supposed to be smarter than his assistant.

In the story, Aminadab is supposed to be pretty dumb.  The description of Aminadab makes it clear that he is not really all that smart and Aylmer definitely treats him that way.  Yet, from the beginning, Aminadab thinks that it is a bad idea to try to remove the birthmark.

As it turns out, Aylmer was wrong -- he was unable to remove the mark without killing Georgiana.  So the intellectual man did not understand what the dumb man did.  That is ironic.