Anne of the Thousand Days explores the perilous path of unchecked ambition through its central characters, Anne and Henry. Both characters become ensnared by desires that are either beyond their reach or morally questionable. Their relentless pursuit of these desires leads them to commit egregious acts, sealing their fate in a historical eternity marred by infamy. In their quest to satisfy sexual, political, and material cravings, they abandon everything of genuine worth, ultimately binding themselves to a shared legacy of ruin.

From the very beginning, the protagonists in the narrative are entangled in their own imperfections, setting the stage for unavoidable conflict. It is Henry who first propels the drama forward. As a king, he relishes the extensive powers of royalty, aligning perfectly with his exaggerated sense of masculinity and pride. To him, any unconquered territory signifies a challenge, and Anne's initial resistance only fuels his determination. In a retaliatory maneuver, Henry takes from Anne the man she truly desires, igniting a flame of vengeful ambition in them both. Their relationship, founded on mutual denial of the simple pleasures they originally sought, evolves into a tumultuous and interdependent bond, spiraling towards inevitable catastrophe.

Their passionate and violent romance is marked by audacious promises neither can wholly fulfill. Henry assures Anne of a crown, while Anne pledges to provide him with a son. Although technically within Henry's power to crown whom he wishes, the path to making Anne his queen demands a heavy toll, exacting a bloody price from his soul. Anne's vow to bear a son defies nature itself, a promise she ultimately cannot keep. Her failure, unlike Henry's, is a mortal one, yet in the throes of her downfall, Anne perceives the folly of her ways. As she faces her demise, she achieves a state of moral transcendence.

The high-stakes game between Henry and Anne is driven by their indomitable personalities and fierce ambitions. It starts as a battle of wills between two potent forces but quickly escalates to a realm where justice is suspended and tyranny reigns. Their actions lead to a profound spiritual upheaval and a transformation of their world, leaving an indelible mark on history. Their relentless pursuit of power and desire constructs a legacy of monumental change: "What we were will be permanent in England, however it came about, whether your will, or mine, or theirs." Through their ambition, they etch their influence permanently into the fabric of England, underscoring the irrevocable impact of their decisions.