Why does Aylmer's assistant, Aminadab, laugh the second time at the end of "The Birthmark"?

Aylmer's assistant, Aminadab, laughs for the second time at the end of "The Birthmark" because he is laughing at the irony of Aylmer's obsession with the superficial, physical aspect of Georgiana. Despite being a spiritually refined man, he is unable to appreciate his wife's spiritual characteristics, and his obsession for her perfection results in her complete destruction.

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When Aylmer's assistant Aminadab is introduced, Hawthorne describes him as symbolizing the purely physical aspect of life, whereas Aylmer stands for the spiritual:

With his vast strength, his shaggy hair, his smoky aspect, and the indescribable earthiness that incrusted him, he seemed to represent man's physical nature; while Aylmer's slender...

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When Aylmer's assistant Aminadab is introduced, Hawthorne describes him as symbolizing the purely physical aspect of life, whereas Aylmer stands for the spiritual:

With his vast strength, his shaggy hair, his smoky aspect, and the indescribable earthiness that incrusted him, he seemed to represent man's physical nature; while Aylmer's slender figure, and pale, intellectual face, were no less apt a type of the spiritual element.

Aminadab is not merely physical but bestial. When he speaks (which he seldom does) his tones are "more like the grunt or growl of a brute than human speech." It is unsurprising, therefore that his laughter is "a gross, hoarse chuckle" and that it has a symbolic meaning, connecting with his bestial nature at the end of the story. Aminadab's second laugh, immediately after Georgiana dies, is described in the following terms:

Then a hoarse, chuckling laugh was heard again! Thus ever does the gross fatality of earth exult in its invariable triumph over the immortal essence which, in this dim sphere of half development, demands the completeness of a higher state.

This laughter might be regarded as demonic if demons were not more spiritual than Aminadab. It is a laugh of malicious triumph at the victory of the gross, physical fact of death over the beauty of Georgiana's spirit. Although Aylmer is described as being "a type of the spiritual element," it is his obsession with the physical detail of the birthmark that has led directly to his wife's death. At first glance, Aylmer seems like a spiritually developed character, certainly in comparison with his gross, bestial assistant. In fact, however, he has thrown away Georgiana's life and his own happiness because he cares too much about physical perfection and fails to appreciate his wife's spiritual qualities. This means that Aminadab's symbolic victory of the physical over the spiritual encompasses both husband and wife.

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