The characters in Charlotte's Web include both animals and humans. Some important animal characters are Wilbur, Charlotte, Templeton, and Uncle. Some important human characters are John, Fern, Avery, Henry, and Homer.

Wilbur is a pig and the runt of his litter. He is adopted by Fern, who cares for him until he is larger. At that point, he goes to live on the Zuckerman farm. He befriends Charlotte and Templeton. Gaining fame from the webs Charlotte spins with English words on them, he is later entered in the fair. He does not win the normal prize, but gets a special one acknowledging him as a famous pig—thus his life is spared from slaughter. He lives the rest of his life on the Zuckerman farm with many generations of Charlotte's descendants.

Charlotte is a spider who lives on the Zuckerman farm. Maternal and wise, she decides to save Wilbur from slaughter by praising him on her web. This excites people and leads to Wilbur's entry in the fair. Charlotte lays an egg sac at the fair but dies once Wilbur's life has been spared. 

Templeton is a rat who lives on the Zuckerman farm. He loves to eat. He is friends with Charlotte and Wilbur. He makes a trip to the dump to find pages with words that Charlotte can use to write on her web.

Uncle is the large pig who wins the prize at the fair Wilbur was hoping to win.

Nellie, Joy, and Arenea are first generation descendants of Charlotte who stay at the farm and become friends with Wilbur.

John Arable is the father of Fern and Avery. He gives Wilbur to Fern at the beginning of the story. He later sells him to Fern's uncle so that she can continue to see Wilbur.

Fern Arable is Wilbur's owner. She loves him deeply and can understand the animals when they speak. She nurses him from being the runt of his litter to being a much larger pig and continues to visit him at the Zuckerman farm.

Avery Arable is Fern's older brother.

Homer Zuckerman is Fern's uncle and the owner of the farm where Wilbur, Charlotte, and Templeton live. He is the one who enters Wilbur in the fair.

Henry Fussy is a boy who Fern develops feelings for during the story as she gets older. She spends time with him at the fair.

There are many different human and animal characters in E.B. White's animal fable Charlotte's Web. Here is a list and short description of the characters in the novel.

Wilbur- The main character who begins life as a runt. The novel circles around the idea if Wilbur will live a long life or die as farm pigs are meant to.

Charlotte- A spider who weaves webs in order to try to save Wilbur's life.

Fern Arable- The little girl who saves Wilbur from being killed because he is a runt. Fern can hear Wilbur and the other animals speak.

Henry Fussy- Fern's friend who she begins to have feelings for (by the end of the novel).

Homer Zuckerman- Fern's uncle who keeps Wilbur after Fern can no longer keep him. Homer takes Wilbur to fairs and promotes him as Charlotte weaves sayings into her web.

Templeton- Templeton is a rat who lives on the Zuckerman farm. Charlotte bribes him into helping the fight for Wilbur's life.

Uncle- Uncle is the pig at the fair who Wilbur thinks he has to beat in order to live. Uncle wins the "Best Pig" prize, but Wilbur wins one as well.

There are also horses, geese, sheep, and a lamb who sporadically speak to Wilbur.