Which characters appear to be the antagonists to Macbeth in Act Three?

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Banquo appears to Macbeth to be a chief antagonist in Act III--and not without reason, as Banquo has started to suspect Macbeth had a hand in Duncan's murder. For Macbeth, the main cause of worry is the witches's prophecy that Banquo's descendants will gain the Scottish throne. Now that he has the throne, it is no longer enough for Macbeth himself to be king. Given the enormity of the price has had paid, he doesn't feel the throne is worth it if he can't pass it to his own heirs. Macbeth, therefore, has Banquo killed.

Meanwhile, a more dangerous antagonist emerges in Act III. This is Hecate, the head of the witches. She confronts the three witches from Act I, angry that they prophesied to Macbeth without consulting her. She accuses them of being too kind to him, saying he has no interest in or sympathy with the witches at all. Why help him, she says, when he won't return the favor? She is highly antagonist to Macbeth and commands the witches to deceive him and lead him to his downfall. She is a bigger threat to Macbeth than Banquo, but Macbeth doesn't realize this, continuing to trust in the witches's prophecies.

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In Act 3, Macbeth has been crowned king, and instead of allaying his fears and his ambitions, his new crown is only increasing both. He begins especially to worry about Banquo, who becomes his antagonist in this act. He finds himself unsatisfied in his new position as king because he still feels threatened: "To be thus is nothing, but to be safely thus" [emphasis mine]. The witches prophesied that Banquo's children would eventually become kings themselves, and that means that Macbeth will be unable to pass his crown to his own children—should he have them—and could possibly mean that Banquo's son Fleance will one day overthrow him. Macbeth sees it clearly: Banquo is now his enemy, and he and his son have to die. Unbeknownst to Macbeth, Banquo himself has also begun to suspect that Macbeth may have had a hand in Duncan's murder. Macbeth sends men to dispatch his new chief antagonists, but Banquo will still manage to haunt him—literally.

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