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She means that she has no choice but to make her own decision and take her life (literally) into her own hands.
Juliet says 'My dismal scene I needs must act alone.' or in other words, 'I must act my dreadful scene alone.' This is said as Juliet prepares to drink the potion.
She means that since she is about to drink the potion Friar Lawrence gave her, she must act alone in commiting her suicidle act.
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