The turning point of the story The Nightingale and the Rose is when the professor's daughter rejects the student's red rose and, in turn, the student throws the red rose away in the gutter, with a passing cart running over it.
The Nightingale's ultimate sacrifice was to color a white rose into red by using his own blood, sacrificing his life in the name of the love that the student feels for the professor's vain daughter. That part of the story could be deemed as the climax, because the ultimate action was finally achieved after the nightingale asked nearly everywhere for a red rose, without being able to find it.
Yet, we all expect this great sacrifice to be equally rewarded by the undying love of the main couple. Yet, in a dramatic twist, the exact opposite happens: The girl finds the red rose almost insulting, since she was preferred jewels and gifts. Moreover, the boy, far from suffering her ungratefulness, proves to be ungrateful himself by tossing the flower aside and declaring that logic is greater than love.
Hence, the nightingale not only sacrificed his life in vain, but also love, as an ideal, becomes a byproduct of immaturity and not the greatest and most powerful force that the nightingale thought it to be.
The daughter rejects the rose.