Segregation against the Jews is an important theme to be looked at in the play and among all the quotes, Shylock's famous monologue shows one of the most explicitly presented emotions throughout the play
: I am a Jew. Hath not a Jew eyes? Hath not a Jew hands, organs, dimensions, senses, affections, passions; fed with the same food, hurt with the same weapons, subject to the same diseases, heal'd by the same means, warm'd and cool'd by the same winter and summer, as a Christian is? If you prick us, do we not bleed? If you tickle us, do we not laugh? If you poison us, do we not die? And if you wrong us, do we not revenge? If we are like you in the rest, we will resemble you in that.
The significance of the quote is that he pledges for equal treatment that every individual deserves despite their personal religious doctrine.