In my mind, the earliest example of how Creon's actions begin to alienate those around him and in the community appears in scene IV. When Haemon tries to convince his father not to kill Antigone, he is unsuccessful. He then vows to leave his father and never see him again. This becomes the first moment where the audience and members of the Chorus are introduced to the idea that Creon is on a collision course with tragic consequences and that he has begun the process of alienation that will become more evident in the later scenes. The chorus of elders in scene V mourn for Antigone, also. This is another example of how Creon is losing the trust of those around him, as their mourning for Antigone is a direct affront to Creon and done so in direct violation of Creon's wishes and his desire to punish Antigone. The elders have distanced themselves from Creon, and this is something he recognizes in the next scene.