First of all, I found it interesting that Robert Frost said so much in such a relatively short poem - in only 20 lines - compared to some other works of his. The poem deals with the paths, the roads, we take in life. The poem is all about choices and how one road leads to one future, while another road can lead to a different future with different people in our circle of influence.
I find the opening line of the poem memorable:
Two roads diverged in a yellow wood,
I find that this unique opening line foreshadows Frost's thoughts for the rest of the poem. Immediately, this opening line compels me to read on to find out what is intriguing about these two roads.
The most memorable line to me, however, is the line:
I took the one less traveled by,
To me, Robert Frost is talking about taking up new challenges in life - going the route people may not typically take. This line alludes to trying new things and meeting new challenges head on. He says that taking the road less traveled has made "all the difference"; he does not say whether the difference has been better or worse for him in his life.
The fact is, he took a chance, maybe even a calculated risk, to choose the road less traveled, and can hold his head high in this decision. He did not waver at the junction of the two roads that diverged. He made a choice, and moved forward with his decision, and lived his life, instead of fearfully failing to take the initiative.
I think this is the most memorable thing I take away from this poem. Frost indicates that he is sorry he could not travel both roads in his life. He understands that we must make hard decisions in life and oftentimes choose one path over another - knowing we can never return to check out the other path. This is where we must have courage and stick to our beliefs, goals, convictions, and plans.