In literature, a struggle or disagreement between two or more contrasting forces in known as conflict. There are several different types of conflict commonly seen in literary works, including character versus character, character versus self, and character versus nature. These three types of literary conflict are demonstrated in Agatha Christie's And Then There Were None.

The overarching conflict of the novel is an example of character versus character conflict: ten strangers are summoned to a remote island by a mysterious, unknown person who accuses them all of murder. One by one, the strangers are subsequently killed in accordance to a children's rhyme. Lesser examples of character versus character conflict are seen in the novel as the ten strangers accuse each other of murder and argue over how to handle their predicament.

All of the ten strangers experience character versus self conflict. Each of them has a dark secret. Everyone who was invited to the island killed at least one person, either directly or indirectly, at some point in their lives.

Nature is the cause of another type of conflict seen in the novel: character versus nature. The book takes place in a mansion located on Soldier Island. The island is completely isolated and surrounded by the ocean on all sides. This setting creates a conflict because it traps the characters on the island and leaves them with no possible escape from the killer.