If you are asking about attacks on the resources needed by the US for making the atomic bomb, the answer is no.  The Manhattan Project was contained within the continental United States and so was not subject to being attacked by any enemy.  Most of the uranium needed came from the US and Canada.  It would have been possible for an attempt to be made to have infiltrators sabotage the project, but no such attempt was ever made.

If you are talking about attacks on other countries' efforts to make an atomic bomb, there were.  The British launched a series of attacks on a plant in Norway that was being used to make "heavy water," which the Germans were trying to use to create an atomic bomb.