I am looking for a method to test the effectiveness of natural antibacterial materials (garlic, tumeric...) against regular disinfectants.
I need to figure out a method in which to do this. I want to test the different disinfectants (natural and unnatural) with all four general types of bacteria and I want to be able to measure its effectiveness in quantitative methods (NOT qualitative) so I can compare them properly. Any ideas?
By the way, this is for my science experiment.
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Here is a possibility. You can use bacterial culturing dishes containing agar gel. Agar is a gelatinous polymer that is a perfect medium for growing bacteria. You can get a sample of various bacteria from different sources using a cotton swab. Swab the surface containing the bacteria and then run the swab across the agar gel. If you let it sit in an incubator (or any warm, still area), the bacteria colony will grow in the dish. To test the effectiveness of the different antibacterial agents, put a small amount on the gel on top of the growing bacteria and let it sit and incubate for some time. You can then see how large of an area of non-bacterial growth there is around each of the different antibacterial agents. If you can find a way to put down an equal amount of each agent, then measuring the size of the non-bacterial area surrounding it would give you a quantitative measure of the effectiveness of each one.
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