When Lady Macbeth asks the spirits to unsex her, she is also
contibuting to the theme/idea of role reversals in the play.
She wants to behave more like a man, and Macbeth, in Act I and
early in Act II, behaves more like what is normally expected of a
female, showing guilt, pity, and mercy. Also, the witches
appear to be women, but have beards. That's role reversal for
dstuva is providing you with two worthy ideas, here. Along
with the role reversal, you can, perhaps, draw parallels to the
"fair is foul, foul is fair" theme (Is not this foul that a
woman wants to be unsexed?) which extends into other areas as
Check out the enotes themes page on Macbeth as well as the
character analysis page. Good luck!