How does Thin Layer Chromatography separate different constituents of a substance? Theoretically explain.
Chromatography is a widely used laboratory technique of separating mixtures. Basically all types of chromatography have the same theory. There is a stationary phase and a mobile phase. The mobile phase is made to run over the stationary phase along with our given mixture. The separation of the given mixture into its constituent parts takes place because of the difference in the affinities of the mixture constituents towards the mobile as well as the stationary phase. This means that the mixture constituent that shows higher affinity towards...
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