1 Answer | Add Yours
Both precipitation and agglutination are immunoassay techniques that involve using the antigen - antibody complex to identify and separate proteins.
For immunoprecipitation, one must first label the sample by adding a marker element or radioisotope. The sample is then incubated with the antigen, and antigen - antibody complexes are allowed to form. The complexes are then passed across a separation plate which contains a bound protein which has a high affinity for the antibody - antigen complexes. The complexes are separated from the bound protein by centrifuging, heating to disrupt the protein, or pH adjustment, and are collected.
Agglutination is a simpler version of the same technique. To do it you mix an agglutinin with a liquid blood sample, and if the corresponding protein is present in the sample, you will see clumping. It is useful as a rapid diagnostic test for things such as blood group typing.
If you need more details on the techniques, you will find an excellent reference from Tulane University here.
We’ve answered 318,989 questions. We can answer yours, too.Ask a question