The Protestant Reformation caused certain countries and principalities to break away from the Roman Catholic Church, ending the power of Rome over principalities in Germany, as well as in Scandinavia, Switzerland, and Great Britain. This led to changes in daily life: for example, people were able to read the Bible in their own languages, as the Bible was translated from Latin into vernacular languages. In everyday life, the newly minted Protestants could read the Bible for themselves, think for themselves, and perform their own "examens" of conscience without having to rely on confessing to a priest.
In England, Henry VIII broke away from the Catholic church so that he could divorce his first wife and marry Anne Boleyn. When Anne Boleyn's daughter, Elizabeth I, became a long-reigning queen, Protestantism won as the entrenched religion of the land.
Although James I, Elizabeth's successor, was more papist in his leanings than Elizabeth, he remained in the Church of England. However, he increased the persecution of Protestant sects that existed outside of the official state church, which Elizabeth had tried harder (if not always successfully) to tolerate. The Reformation both allowed these radical sects to spring up and kept James I from the severe persecution implemented by the Inquisition in Catholic territories. This had a direct impact on the colonization of North America. The Protestant and Puritan groups who believed that the English Reformation had not gone far enough to purify the Church were persecuted to an extent that made staying in England untenable, but they were not wiped out. This led to a solution that suited everyone: the establishment of colonies, which removed problematic religious groups, such as Puritans and Quakers, from the British isles, putting an ocean between their troublesome ideas and their home country.
The Reformation, therefore, indirectly impacted the direction and tenor of colonization in at least some of the American colonies.