Explain the following Explain why - the presence of protease and lipase would make washing powder more effective than ordinary detergent Explain why - the washing powder should not be used in boiling water

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The commonest stains and smudges on clothing and other fabrics are usually either oil based or protein based. Oil based stains such as grease spots, makeup smears, chocolate, and egg yolk will not come out easily with ordinary soap. A combination of warm water to soften the oil and an enzyme called lipase, which breaks down the structure of oil, will usually be needed to remove the stain.

Protein based stains such as blood, dairy products, mud, or feces are better washed in cold water (heat tends to set protein stains) with an enzyme called protease, which breaks down the structure of proteins.

The enzymes lipase and protease are themselves proteins, and exposure to high heat will cause their structure to change, a process called denaturation, which renders them ineffective. If you use excessively hot water to do the laundry, the enzymes will denature and they will not work, and the heat will make some of the stains on the fabric become permanent as well.


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Protease and lipase help break down amino acids and fats, respectively. Their presence will help break down food stains into compounds that are more easily washed away with the soaps present in the washing powder. Ordinary detergent, without these additives, cannot always remove certain food stains so this should result in cleaner clothes.

Enzymes lose their functionality at higher temperatures because they begin to lose their tertiary structure which allows them to interact with the compounds in order to break them down (see link below). When an enzyme is denatured (becomes unreactive), then it will no longer serve the same function. Enzyme function depends not only on the specific chain of amino acids used to assemble it but also on it's secondary structure (like the helix structure of DNA) but it's tertiary structure (the loops, bends, and curves that determine the "big picture" shape of the molecule.

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