In e. coli genomic dna isolation, if we add sds solution excess by mistake, what will happen?what are the reasons behind there to get a unclear solution thats means wrong result or colloidal...

In e. coli genomic dna isolation, if we add sds solution excess by mistake, what will happen?

what are the reasons behind there to get a unclear solution thats means wrong result or colloidal solution

 

Asked on by jshree005

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bandmanjoe | Middle School Teacher | (Level 2) Senior Educator

Posted on

The SDS solution is used to give the different proteins in the e. coli different charges, so they will line up in the stacking gel according to their repective positive and negative charges.  If the solution is cloudy or unclear, with little separation, it probably means not enough SDS solution was added to achieve the separation you were looking for.  So the addition of SDS in excess should achieve some degree of separation.

E. coli is a common form of coliform bacteria that can flourish in the human intestine.  It can exist with oxygen or without oxygen, aerobic or nonaerobic.  It can cause diahrrea, and can be fatal in afflictions among children.  E. coli is a gram-negative type of bacteria, immersion in an SDS solution should cause significant banding within the gel stack of the medium.

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