Since the emergence of an industrial society, has there been an alteration in which people are losing the spiritual connection they once had with work?
It is hard to say if this is the case since there were no opinion polls taken of workers in the days before society became industrialized. Therefore, we cannot know how the mass of people experience this change. However, many people (including Karl Marx) believe that industrialization does do this.
The reasoning is that people in an industrial society are not their own bosses. They do not do skilled and satisfying work at their own pace. Instead, they work when they are told to work and often do jobs (as in factories) that take little skill. This makes their work more of a "job" than a "calling."
However, it seems somewhat unlikely that most people in pre-industrial times had a "spiritual connection" with their work. Most people had to work very hard from morning to night trying to grow enough food to stay alive. This may seem spiritual and romantic in retrospect, but it is hard to say whether the people in those times really experienced it in that way.