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If you are referring to the murder of Hamlet Sr., it is certainly not stated explicitly that she knew anything about it or even suspected it. Hamlet makes some reference to it, calling Claudius a murderer but does not go into it further. Of course, Gertrude doesn't call him on this either so perhaps she is in some ways indicating that she understands Hamlet's feelings about it?
When Hamlet refers to Claudius as "A murderer and a villain, A slave that is not twentieth part the tithe of your precent Lord," Gertrude says only "No more." but it would be difficult to construe this as an indication that she was aware of Hamlet Sr.'s murder.
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