To answer the following questions, refer to this template DNA sequence: ATACCGTTATTTAACCGCCAGAAT What is the sequence of the non-coding (non-template) DNA strand? What is the mRNA sequence (transcription)?
You are only allowed to ask one question so I have edited down. The first two questions are very closely linked so I have left them as shown.
When given a template sequence of DNA that is to be read for protein coding (transcription), we know that the strand is naturally found as part of a double helix. That means that there is a complementary sequence to the template sequence found in the DNA. In DNA, G binds to C and A binds to T (and vice versa). So the sequence of the non-coding strand would be: TATGGCAATAAATTGGCGGTCTTA.
When transcription takes place, the strand of DNA is read by RNA polymerase and a complementary strand of mRNA is created. The difference here is that with RNA, T is replaced by U. So the sequence of mRNA created would be: UAUGGCAAUAAAUUGGCGGUCUUA.
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