The charge of the nucleus of an atom with 25 protons is +25 regardless of the number of other particles. Protons are the only charged particle in the nucleus. However, the overall charge of the atom is zero. That's because protons in the nucleus and electrons outside the nucleus have opposite charges and the sum of +25 and -25 is zero. The nuclear charge of any atom is given by its atomic number.
Here are the charges, locations and approximate masses of the sub-atomic particles:
proton - in nucleus, charge = +1, mass = approximately 1 amu
neutron - in nucleus, charge = 0 (neutral), mass = approximately 1 amu
electron - outside nucleus, charge = -1, mass = approximately 0 amu
(The mass of the electron is often neglected when calculating the mass of an atom. It has a value of 5.49 x 10^(-4) amu, or 1/1840 of an amu.)