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While isolating DNA from plant cell,which DNA gets isolated out of these:Mitochondrial...

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cute1590 | Student, Undergraduate | eNotes Newbie

Posted July 7, 2011 at 11:36 PM via web

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While isolating DNA from plant cell,which DNA gets isolated out of these:Mitochondrial DNA or Chloroplast or nuclear DNA?

And what about DNA from blood?

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pacorz | High School Teacher | (Level 3) Educator

Posted July 13, 2011 at 11:07 AM (Answer #1)

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In plants, the chloroplasts and the mitochondria have their own DNA independent of the rest of the cell's DNA. This is inherited only from the female parent; hence if you want a DNA sample that represents the plant and which derives from both the female and male parents, you need to get a nuclear DNA sample.

In blood, the vast majority of cells in a sample are erythrocytes, or red blood cells, which have no nucleus at maturity. To get a representative sample of DNA from blood, you should centrifuge the blood sample to separate its conponents, and get the DNA from the leuukocyte, or white cell, portion, which is where nuclear DNA will be found.

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