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What is mass analysis in Physics?   Eg - Accelertor Mass Spectrometry differs from...

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astrosonuthird | Student | (Level 1) Valedictorian

Posted July 3, 2013 at 10:07 AM via web

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What is mass analysis in Physics?

 

Eg - Accelertor Mass Spectrometry differs from other forms of mass spectrometry in that it accelerates ions to extraordinary high kinetic energies before mass analysis.

 

Can anyone explain this para especially the MASS ANALYSIS?

 
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jerichorayel | College Teacher | (Level 1) Senior Educator

Posted July 3, 2013 at 6:20 PM (Answer #1)

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Mass analysis is a term generally used to describe a method of determining quantitatively the amount of a substance or sample. The data received are typically fragments of a particular sample that is generated through a spectrum in order to analyze the elemental contents of the samples. There are many ways in determining the mass of a particular sample through mass analysis but basically they are under certain mechanism general to all. 

1. The sample is ionized; usually into cation form by an ion source.
2. The ions formed are separated based on their mass and charge. For example, Sample A is split into B, C and D fragments. These three fragments have different characteristic and masses that is detected by the Detector.
3. The detector separate, measure and place them in the scale so as to create a chart which is apparently your spectrum. 

Considering the image you just uploaded, that method of mass analysis is called the Matrix-assisted laser desorption/ionization - time of flight mass spectroscopy or the MALDI-TOF. This method allows the soft ionization of samples. When we say soft, it means that the ionization of the sample doesn't lead to numerous fragmentations of the samples. This is a very good method in quantitatively determining biomolecules such as proteins, DNA, sugars and other polymers. 

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