what is the best method to conduct hemolytic test of lactobacillus to be used as probiotic in human's food?
please state in your advise or reply answer on what type of blood is best used if hemolytic test is to be conduct using blood agar.
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Normally sheep blood agar (5%) is used in the detection of hemolytic Lactobacillus species. A positive test will reveal pinpoint alpha hemolytic colonies on this agar. The appearance of alpha hemolysis will be a zone of intact but discolored (usually greenish or brownish) erythrocytes around the colony. This occurs because of the action of peroxide secreted by the bacteria.
Many species of Lactobacillus show no hemolytic capability, which of course should be the first selection criterion for introduction into the human diet. Lactobacillus would be a good candidate for inclusion as a human probiotic because some species, notably L. casei and L. paracasei, have shown inhibitory activity against Staphylococcus aureus.
The second link below has the formula for preparing sheep blood agar.
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