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What approach would you use to isolate only cells that contain this new structure that...
What approach would you use to isolate only cells that contain this new structure that is bound to the interior surface of the cell membrane as described below?
In a newly-explored deep-sea environment, several potentially new species of apparent bacteria have been discovered. In some of these single-celled life forms, a novel structure has been observed. The structure is apparently bound to the interior surface of the cell membrane and so does not float freely in the cytosol. Preliminary investigation indicates that this structure typically appears circular or ovoid in shape. It apparently consists of a long chain of nucleic acid wrapped around a tube of an unknown protein.
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Isolation of different cells depends on the structure of the outside cell wall. Several cell cultures are used to develop in order to selectively classify cells from each other.
First, determine the environment of the bacteria where it is found. It would be better if the bacteria to be gathered are alive and reproducing before doing further experiments. Analysis of the water sample should be conducted so making the culture dish possible for the unknown species to grow.
Second, cells may be different depending on the extracellular matrix and will be the key in the separation. There are methods that are used in order to separate them based on the outer cellular structure. These are the fluorescence-activated cell sorter, a method that separates cells based on charges; microdissection, and usage of antibodies.
The unknown protein and the novel structure can be identified by first separation from the entire cell contents, probably by centrifugation or digestion and then analysed through spectroscopic methods.
Posted by jerichorayel on August 26, 2013 at 8:32 AM (Answer #1)
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