How would you mix the .5M glucose solution you just made with distilled water to make 100ml of a 0.05 M glucose solution?



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This is a dilution problem wherein we are looking how much volume of the stock solution we need to dilute. We have:

`M_1V_1 = M_2V_2`

`M_1` = 0.5 M

`V_1` = ?

`M_2 ` = 0.05 M

`V_2` = 100mL = 0.100L *

`M_1V_1 = M_2V_2`

`(0.5)*V_1 = (0.05)*(0.100)`

`V_1 = ((0.05)*(0.100))/0.5`

`V_1 = 0.01 = 10 mL`


Total volume = volume of Sample + volume of diluent

100 mL = 10 mL + volume of diluent

Volume of diluent = 100-10 =90 mL


Answer: To prepare the said solution, we need to measure 10mL of 1M glucose solution and place it in a flask. Add 90 mL of distilled water and stir the solution.


*For proper computation, the volume units is converted from mL to L. 1000 mL = 1L


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