1 Answer | Add Yours
This process comprises three steps: (1) recognition of the origin B protein(i.e., 5'-3' helicase; also acts as the activator of primase). Thus, Dna-A (or initiator protein) ATP complex binds at 9bp inverted repeat regions (R1, R2, R3, R4) of ori C of E.coli and promotes popening of the DNA duplex in a region og three direct repeats of 13-bp sequence called 13-mers. The opening occurs from right 13-mer leftwards and requires negatively supercoiled DNA and HU or IHF initiator proteins. Dna B is transferred to exposed single stranded DNA and causes unwinding of the DNA in the presence of ATP, SSB protein and DNA gyrase.
We’ve answered 330,328 questions. We can answer yours, too.Ask a question