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Enzymes are organic catalysts. They facilitate chemical reactions. If an enzyme is mixed with compounds A, B and C(reactants) then after an interval of five minutes, the concentration of the product(P)could be measured. The problem of this experiment is at what temperature does this particular enzyme work optimally? The hypothesis could be--the enzyme works best at 37 degrees Celsius. Whatever the hypothesis is, the chemical reaction can proceed at various temperatures--above or below 37 degrees Celsius as well as at 37 degrees. By collecting the data at each temperature for how much product is produced, one can see what the optimal temperature for this enzyme is.
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